LOCUS pnpaw2026 7644 bp DNA circular SYN 11-JUL-2026 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7644) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 11, 2026 from SnapGene 8.1.1 https://www.snapgene.com COMMENT Vector for standard cloning, efficient in vitro RNA synthesis, and ssDNA production. Identical to pGEM(R)‑3Zf(-) except for the orientation of the f1 origin. FEATURES Location/Qualifiers source 1..7644 /mol_type="other DNA" /organism="synthetic DNA construct" ORIGIN 1 gggcgaattc gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc 61 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac 121 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat 181 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt 241 gtggaaagga cgaaacaccg ggaacctgat catgtagatc gaatggactc taaatccgtt 301 cagccgggtt agattcccgg ggtttccgcc attttttgag ctcggtaccc ggggatcccg 361 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 421 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 481 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 541 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 601 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 661 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 721 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 781 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac 841 ggtgggaggt ctatataagc agagctgcca ccatggtaag aaaccattga acaccctcat 901 ctctgccact ggcctgtgga tgtccagaac ggggaccatt cacaagatca aacaccacga 961 ggtgtccagg tccaagatct acattgagat ggcctgtggg gaccacctgg tggtgaacaa 1021 ctccagaagc agccgcacag cccgggccct gagacaccac aagtacagaa agacctgcaa 1081 gcgctgccgg gtgtctgatg aggacctgaa caagttcctg accaaggcca atgaggacca 1141 gacatctgtg aaggtgaagg tggtgtctgc ccccaccaga accaagaaag ccatgcccaa 1201 atctgtggcc cgggccccca agcccctgga gaacacagag gctgcccagg cccagccctc 1261 agggtccaag ttctctcctg ccatccctgt gtccacccag gagtctgtgt ctgtgcctgc 1321 ctctgtgtcc acctccatct cctccatctc cactggagcc actgcatctg ccctggtgaa 1381 gggcaacacc aaccccatca cctccatgtc tgcacctgtg caggcctctg cccctgccct 1441 gaccaagagc cagacagacc gcctggaggt gctgctgaac cccaaggatg agatcagcct 1501 gaactcaggg aagcccttcc gggagctgga gagcgagctg ctgagccgca ggaagaagga 1561 cctgcagcag atctatgctg aggagcggga gaactacctg gggaagctgg agcgggagat 1621 cacccgcttc tttgtggacc ggggcttcct ggagatcaag agccccatcc tgatccccct 1681 ggagtacatt gagagaatgg gcattgacaa tgacactgag ctgagcaagc agatcttccg 1741 ggtggacaag aacttctgcc tgcggccaat gctggccccc aacctctaca actacctgag 1801 gaagctggac cgggctctgc ctgaccccat caagatcttt gagattgggc cctgctacag 1861 gaaggagagt gatgggaagg agcacctgga ggagttcacc atgctgaact tctgccagat 1921 gggctctggc tgcacccggg agaacctgga gtccatcatc actgacttcc tgaaccacct 1981 ggggattgac ttcaagattg tgggggacag ctgtatggtg tatggggaca ccctggatgt 2041 gatgcatggg gacctggagc tgagctctgc tgtggtgggg cccattcccc tggaccggga 2101 gtggggcatt gacaagccct ggattggggc tggctttggc ctggagcggc tgctgaaggt 2161 gaaacatgac ttcaagaata tcaagagagc tgcacggtct gagagctatt acaatggcat 2221 ctccacaaac ttaggcggcg gaggctcagg tggagggggc agtgggggtg gcggctcagg 2281 aggaagcggt ggacgtagtg ggggaggtag cggccccaga cctagaggca cccgggggaa 2341 aggccgtaga atccgccggc gggggtaagc taactgaaac acggaaggag acaataccgg 2401 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt 2461 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgattct agagtcgacc 2521 tgcaggcatg cggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 2581 agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa 2641 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 2701 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 2761 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg 2821 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg 2881 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag 2941 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct 3001 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc 3061 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg 3121 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc 3181 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg 3241 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc 3301 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct 3361 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa 3421 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca 3481 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt 3541 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc 3601 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca 3661 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga gccaccatga 3721 actgcacccg cgaggagcac tagtgatccg agggctccgg ctacccctac gacgtgcccg 3781 actacgccta aggcgcactg gcgctgcgcc ttcgggcgcc aatcgtagcg tgtcggcgcc 3841 catcctgcca ggtctcctgg agccatgatg acaacggccc tagatcactt cccacccctc 3901 tgacaggacc tctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 3961 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 4021 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 4081 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 4141 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 4201 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 4261 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt 4321 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 4381 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 4441 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 4501 gcaagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg 4561 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 4621 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 4681 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 4741 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 4801 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4861 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4921 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 4981 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 5041 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 5101 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 5161 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 5221 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 5281 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 5341 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 5401 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 5461 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 5521 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 5581 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 5641 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 5701 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 5761 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 5821 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 5881 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 5941 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 6001 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 6061 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 6121 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 6181 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 6241 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 6301 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 6361 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 6421 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 6481 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 6541 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 6601 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 6661 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 6721 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt 6781 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 6841 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 6901 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 6961 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg 7021 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat 7081 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg 7141 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc 7201 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt 7261 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 7321 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 7381 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 7441 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg 7501 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 7561 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 7621 tgaattgtaa tacgactcac tata //